在(zai)人(ren)(ren)類(lei)(lei)疾(ji)病(bing)(bing)(bing)研(yan)(yan)究(jiu)中數據(ju)表(biao)明，常(chang)用(yong)(yong)實驗動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)按(an)產生(sheng)(sheng)原因(yin)(yin)分為以(yi)(yi)下(xia)(xia)5類(lei)(lei)：自發(fa)性(xing)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)、誘(you)發(fa)型(xing)(xing)(xing)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)、遺(yi)(yi)傳(chuan)工(gong)(gong)程(cheng)(cheng)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)醫(yi)學(xue)(xue)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)和陰性(xing)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。下(xia)(xia)面(mian)上海研(yan)(yan)錄(lu)帶大家一(yi)起看看吧：1、自發(fa)性(xing)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)：是指(zhi)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)未(wei)經任何有意識的(de)人(ren)(ren)工(gong)(gong)處(chu)理，在(zai)自然條件下(xia)(xia)或(huo)(huo)基因(yin)(yin)突變條件下(xia)(xia)所產生(sheng)(sheng)的(de)疾(ji)病(bing)(bing)(bing)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。主要包括(kuo)突變型(xing)(xing)(xing)的(de)遺(yi)(yi)傳(chuan)病(bing)(bing)(bing)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)和近郊系(xi)的(de)疾(ji)病(bing)(bing)(bing)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。2、誘(you)發(fa)型(xing)(xing)(xing)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)：亦稱實驗性(xing)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。是使用(yong)(yong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)理、化學(xue)(xue)或(huo)(huo)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)致病(bing)(bing)(bing)因(yin)(yin)素誘(you)導動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)產生(sheng)(sheng)某些類(lei)(lei)似人(ren)(ren)類(lei)(lei)疾(ji)病(bing)(bing)(bing)表(biao)現而制備的(de)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。此模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)具有制備方法(fa)簡(jian)單，實驗條件容易控制，重復性(xing)好(hao)等特(te)(te)點(dian)，廣(guang)泛應(ying)(ying)用(yong)(yong)于藥(yao)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)篩(shai)選、毒理、傳(chuan)染病(bing)(bing)(bing)、病(bing)(bing)(bing)理機制的(de)研(yan)(yan)究(jiu)。3、遺(yi)(yi)傳(chuan)工(gong)(gong)程(cheng)(cheng)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)：是利用(yong)(yong)遺(yi)(yi)傳(chuan)工(gong)(gong)程(cheng)(cheng)技(ji)術(shu)對動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)基因(yin)(yin)組(zu)進行修飾，用(yong)(yong)于研(yan)(yan)究(jiu)基因(yin)(yin)功(gong)能或(huo)(huo)疾(ji)病(bing)(bing)(bing)機制的(de)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。也稱基因(yin)(yin)修飾動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)，是指(zhi)利用(yong)(yong)胚胎工(gong)(gong)程(cheng)(cheng)和基因(yin)(yin)工(gong)(gong)程(cheng)(cheng)等生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)技(ji)術(shu)有目(mu)的(de)的(de)干(gan)預動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)遺(yi)(yi)傳(chuan)組(zu)成(cheng)，導致動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)出現新的(de)性(xing)狀，并(bing)使其(qi)能夠有效地遺(yi)(yi)傳(chuan)下(xia)(xia)去，形(xing)成(cheng)新的(de)可(ke)供生(sheng)(sheng)命科學(xue)(xue)研(yan)(yan)究(jiu)的(de)和其(qi)他目(mu)的(de)所用(yong)(yong)的(de)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。4、生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)醫(yi)學(xue)(xue)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)：也是指(zhi)利用(yong)(yong)健康生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)的(de)特(te)(te)定(ding)生(sheng)(sheng)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)學(xue)(xue)特(te)(te)征(zheng)，研(yan)(yan)究(jiu)人(ren)(ren)類(lei)(lei)疾(ji)病(bing)(bing)(bing)相(xiang)似表(biao)現得(de)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)。這(zhe)類(lei)(lei)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)與人(ren)(ren)類(lei)(lei)疾(ji)病(bing)(bing)(bing)存在(zai)一(yi)定(ding)的(de)差異，研(yan)(yan)究(jiu)者應(ying)(ying)加(jia)以(yi)(yi)比較，從中獲(huo)得(de)有關材(cai)料。C57BL/6成(cheng)年(nian)鼠肺(fei)部通過呼吸機過量通氣損(sun)傷模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)的(de)建(jian)立；云南C57動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)(mo)型(xing)(xing)(xing)手術(shu)

IgA腎(shen)病(bing)(bing)小鼠(shu)模型【目的(de)】建(jian)立IgA腎(shen)病(bing)(bing)小鼠(shu)模型，并觀察(cha)模型的(de)生化及(ji)病(bing)(bing)理(li)指標特點。【材(cai)料】1、酸化水：鹽酸調滅菌(jun)水；2、蓖麻(ma)油+CCl4：5:1配(pei)制；3、LPS：；4、血清(qing)白(bai)(bai)蛋(dan)白(bai)(bai)BSA：800mg/kg用(yong)量，滅菌(jun)水配(pei)制；【方法】小鼠(shu)BSA+CCl4+脂多糖(tang)LPS誘(you)導法1、20g小鼠(shu)牛血清(qing)白(bai)(bai)蛋(dan)白(bai)(bai)BSA(800mg/kg)隔天灌(guan)胃(wei)1次(ci)，配(pei)制160mg/ml，灌(guan)胃(wei)100ul/只，持續8周(zhou)(zhou)(zhou)；2、同時皮下注射(she)蓖麻(ma)油和CCl4（5:1），1次(ci)/周(zhou)(zhou)(zhou)，持續8周(zhou)(zhou)(zhou)；3、分別于(yu)第6、8周(zhou)(zhou)(zhou)以（）尾靜脈注射(she)LPS，1次(ci)/周(zhou)(zhou)(zhou)；4、每兩(liang)周(zhou)(zhou)(zhou)取(qu)24h尿(niao)(niao)液(ye)一次(ci)觀察(cha)尿(niao)(niao)蛋(dan)白(bai)(bai)及(ji)紅細(xi)(xi)胞；5、每日觀察(cha)精(jing)神、飲食、大小便，第9周(zhou)(zhou)(zhou)處死(si)，取(qu)血和腎(shen)、肝做相應檢查；6、取(qu)尿(niao)(niao)液(ye)后2000r/min離(li)心10min，取(qu)上清(qing)用(yong)全自(zi)動生化儀檢測尿(niao)(niao)蛋(dan)白(bai)(bai)肌酐比(bi)值，鏡下觀察(cha)尿(niao)(niao)沉渣中有無紅細(xi)(xi)胞。上海(hai)皮下成瘤(liu)動物(wu)模型技術特發性肺纖維化（IPF）小鼠(shu)模型建(jian)立。

中風模(mo)(mo)型(xing)(xing)）股動(dong)(dong)(dong)脈(mo)(mo)(mo)(mo)內皮損傷模(mo)(mo)型(xing)(xing)頸動(dong)(dong)(dong)脈(mo)(mo)(mo)(mo)結(jie)扎(zha)或部分(fen)結(jie)扎(zha)術(shu)(shu)(shu)(shu)(shu)(shu)深靜(jing)脈(mo)(mo)(mo)(mo)血(xue)(xue)栓模(mo)(mo)型(xing)(xing)下(xia)肢缺血(xue)(xue)模(mo)(mo)型(xing)(xing)門(men)腔靜(jing)脈(mo)(mo)(mo)(mo)分(fen)流術(shu)(shu)(shu)(shu)(shu)(shu)左、右心(xin)血(xue)(xue)流動(dong)(dong)(dong)力學(xue)測(ce)試左心(xin)系(xi)統測(ce)壓(ya)右心(xin)系(xi)統測(ce)壓(ya)腹(fu)部手(shou)(shou)術(shu)(shu)(shu)(shu)(shu)(shu)膽管(guan)(guan)(guan)(guan)(guan)(guan)(guan)結(jie)扎(zha)術(shu)(shu)(shu)(shu)(shu)(shu)胃大部切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)小(xiao)腸(chang)部分(fen)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)急性腎(shen)(shen)衰模(mo)(mo)型(xing)(xing)單側(ce)(ce)腎(shen)(shen)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)3/4腎(shen)(shen)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)5/6腎(shen)(shen)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)脾切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)腎(shen)(shen)上腺(xian)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)腎(shen)(shen)上腺(xian)髓質摘除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)骨關節炎模(mo)(mo)型(xing)(xing)脾神(shen)(shen)(shen)經切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)肝臟迷(mi)走神(shen)(shen)(shen)經切(qie)(qie)(qie)斷(duan)術(shu)(shu)(shu)(shu)(shu)(shu)胃迷(mi)走神(shen)(shen)(shen)經切(qie)(qie)(qie)斷(duan)術(shu)(shu)(shu)(shu)(shu)(shu)膈下(xia)迷(mi)走神(shen)(shen)(shen)經切(qie)(qie)(qie)斷(duan)術(shu)(shu)(shu)(shu)(shu)(shu)去勢(shi)術(shu)(shu)(shu)(shu)(shu)(shu)輸精管(guan)(guan)(guan)(guan)(guan)(guan)(guan)結(jie)扎(zha)術(shu)(shu)(shu)(shu)(shu)(shu)卵巢切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)單側(ce)(ce)或雙側(ce)(ce)輸卵管(guan)(guan)(guan)(guan)(guan)(guan)(guan)結(jie)扎(zha)術(shu)(shu)(shu)(shu)(shu)(shu)子(zi)宮切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)輸尿管(guan)(guan)(guan)(guan)(guan)(guan)(guan)結(jie)扎(zha)術(shu)(shu)(shu)(shu)(shu)(shu)神(shen)(shen)(shen)經系(xi)統手(shou)(shou)術(shu)(shu)(shu)(shu)(shu)(shu)單側(ce)(ce)腦(nao)(nao)(nao)內定位(wei)插(cha)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)雙側(ce)(ce)腦(nao)(nao)(nao)內定位(wei)插(cha)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)側(ce)(ce)腦(nao)(nao)(nao)室(shi)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)側(ce)(ce)腦(nao)(nao)(nao)室(shi)微量滲(shen)透(tou)泵導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)第3腦(nao)(nao)(nao)室(shi)插(cha)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)腓腸(chang)肌神(shen)(shen)(shen)經切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)軟組織手(shou)(shou)術(shu)(shu)(shu)(shu)(shu)(shu)甲狀腺(xian)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)甲狀旁(pang)(pang)腺(xian)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)甲狀腺(xian)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)伴甲狀旁(pang)(pang)腺(xian)再植入(ru)術(shu)(shu)(shu)(shu)(shu)(shu)胸(xiong)腺(xian)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)松果體(ti)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)垂體(ti)切(qie)(qie)(qie)除(chu)(chu)(chu)(chu)(chu)術(shu)(shu)(shu)(shu)(shu)(shu)傳感器植入(ru)手(shou)(shou)術(shu)(shu)(shu)(shu)(shu)(shu)血(xue)(xue)壓(ya)遙(yao)(yao)(yao)(yao)測(ce)左心(xin)室(shi)壓(ya)遙(yao)(yao)(yao)(yao)測(ce)門(men)靜(jing)脈(mo)(mo)(mo)(mo)壓(ya)遙(yao)(yao)(yao)(yao)測(ce)血(xue)(xue)壓(ya)與(yu)心(xin)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)遙(yao)(yao)(yao)(yao)測(ce)血(xue)(xue)壓(ya)與(yu)腦(nao)(nao)(nao)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)遙(yao)(yao)(yao)(yao)測(ce)胸(xiong)內壓(ya)遙(yao)(yao)(yao)(yao)測(ce)胸(xiong)內壓(ya)與(yu)心(xin)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)遙(yao)(yao)(yao)(yao)測(ce)心(xin)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)遙(yao)(yao)(yao)(yao)測(ce)腦(nao)(nao)(nao)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)遙(yao)(yao)(yao)(yao)測(ce)肌電(dian)(dian)(dian)遙(yao)(yao)(yao)(yao)測(ce)心(xin)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)與(yu)腦(nao)(nao)(nao)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)遙(yao)(yao)(yao)(yao)測(ce)腦(nao)(nao)(nao)電(dian)(dian)(dian)圖(tu)(tu)(tu)(tu)與(yu)肌電(dian)(dian)(dian)遙(yao)(yao)(yao)(yao)測(ce)血(xue)(xue)糖遙(yao)(yao)(yao)(yao)測(ce)體(ti)溫與(yu)活動(dong)(dong)(dong)度遙(yao)(yao)(yao)(yao)測(ce)血(xue)(xue)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)手(shou)(shou)術(shu)(shu)(shu)(shu)(shu)(shu)頸動(dong)(dong)(dong)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)顱內給藥頸動(dong)(dong)(dong)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)腹(fu)主動(dong)(dong)(dong)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)股動(dong)(dong)(dong)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)股靜(jing)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)單側(ce)(ce)頸靜(jing)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)雙側(ce)(ce)頸靜(jing)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)門(men)靜(jing)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)下(xia)腔靜(jing)脈(mo)(mo)(mo)(mo)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)非血(xue)(xue)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)手(shou)(shou)術(shu)(shu)(shu)(shu)(shu)(shu)膽管(guan)(guan)(guan)(guan)(guan)(guan)(guan)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)胃導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)十二指腸(chang)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)空腸(chang)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)回腸(chang)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)盲(mang)腸(chang)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)結(jie)腸(chang)導(dao)(dao)(dao)(dao)(dao)管(guan)(guan)(guan)(guan)(guan)(guan)(guan)膀。

可(ke)在(zai)設定的(de)(de)(de)壓(ya)差標(biao)準內(nei)(nei)(nei)無(wu)(wu)極調(diao)控，以保障系(xi)(xi)(xi)統(tong)(tong)(tong)對海拔(3000m～7000m)的(de)(de)(de)微負壓(ya)進行(xing)(xing)模(mo)(mo)擬。并且，進排風風管裝(zhuang)有(you)高(gao)(gao)效(xiao)空氣(qi)(qi)(qi)(qi)(qi)過(guo)濾(lv)器，使進入飼養倉2的(de)(de)(de)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)潔凈。實施(shi)(shi)例(li)(li)3在(zai)實施(shi)(shi)例(li)(li)1的(de)(de)(de)基(ji)礎上提供(gong)的(de)(de)(de)一(yi)(yi)種(zhong)高(gao)(gao)原(yuan)性(xing)(xing)人(ren)類疾病(bing)模(mo)(mo)型制(zhi)備(bei)(bei)(bei)環(huan)境模(mo)(mo)擬系(xi)(xi)(xi)統(tong)(tong)(tong)，所(suo)述(shu)高(gao)(gao)原(yuan)低(di)氧(yang)環(huan)境模(mo)(mo)擬裝(zhuang)置包括(kuo)惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)源(yuan)，所(suo)述(shu)惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)源(yuan)與(yu)(yu)進風系(xi)(xi)(xi)統(tong)(tong)(tong)13連(lian)(lian)接(jie)(jie)，所(suo)述(shu)惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)源(yuan)與(yu)(yu)進風系(xi)(xi)(xi)統(tong)(tong)(tong)13之間設置有(you)比(bi)例(li)(li)式(shi)氣(qi)(qi)(qi)(qi)(qi)閥(fa)，還包括(kuo)設置在(zai)飼養倉2內(nei)(nei)(nei)的(de)(de)(de)嵌入式(shi)氧(yang)測定儀。本(ben)(ben)實施(shi)(shi)例(li)(li)的(de)(de)(de)工(gong)作原(yuan)理：惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)源(yuan)可(ke)以是惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)儲(chu)備(bei)(bei)(bei)罐(guan)或者(zhe)是液態惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)儲(chu)備(bei)(bei)(bei)罐(guan)。在(zai)模(mo)(mo)擬低(di)氧(yang)環(huan)境時(shi)，外(wai)接(jie)(jie)惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)儲(chu)備(bei)(bei)(bei)罐(guan)連(lian)(lian)接(jie)(jie)進風系(xi)(xi)(xi)統(tong)(tong)(tong)13。比(bi)例(li)(li)式(shi)氣(qi)(qi)(qi)(qi)(qi)閥(fa)和嵌入式(shi)氧(yang)測定儀均(jun)與(yu)(yu)功(gong)(gong)能控制(zhi)面板19連(lian)(lian)接(jie)(jie)，通(tong)過(guo)功(gong)(gong)能控制(zhi)面板19上的(de)(de)(de)氧(yang)濃(nong)(nong)度(du)(du)表顯示(shi)濃(nong)(nong)度(du)(du)來(lai)調(diao)節比(bi)例(li)(li)式(shi)氣(qi)(qi)(qi)(qi)(qi)閥(fa)，通(tong)過(guo)加惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)來(lai)來(lai)實現(xian)降低(di)氧(yang)氣(qi)(qi)(qi)(qi)(qi)濃(nong)(nong)度(du)(du)。當(dang)倉體(ti)(ti)(ti)(ti)內(nei)(nei)(nei)的(de)(de)(de)氧(yang)氣(qi)(qi)(qi)(qi)(qi)濃(nong)(nong)度(du)(du)較高(gao)(gao)時(shi)，手動打開(kai)惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)儲(chu)備(bei)(bei)(bei)罐(guan)與(yu)(yu)進風系(xi)(xi)(xi)統(tong)(tong)(tong)之間的(de)(de)(de)氣(qi)(qi)(qi)(qi)(qi)閥(fa)，調(diao)節惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)進入量，使倉體(ti)(ti)(ti)(ti)內(nei)(nei)(nei)氧(yang)氣(qi)(qi)(qi)(qi)(qi)濃(nong)(nong)度(du)(du)降低(di)，觀察控制(zhi)面板上的(de)(de)(de)氧(yang)濃(nong)(nong)度(du)(du)顯示(shi)，調(diao)整氣(qi)(qi)(qi)(qi)(qi)閥(fa)開(kai)度(du)(du)大(da)小，達(da)到需求的(de)(de)(de)氧(yang)濃(nong)(nong)度(du)(du)，以此調(diao)節實現(xian)高(gao)(gao)原(yuan)缺氧(yang)環(huan)境的(de)(de)(de)模(mo)(mo)擬。本(ben)(ben)技術方案采用的(de)(de)(de)惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)為對生命體(ti)(ti)(ti)(ti)無(wu)(wu)危(wei)害的(de)(de)(de)惰(duo)(duo)性(xing)(xing)氣(qi)(qi)(qi)(qi)(qi)體(ti)(ti)(ti)(ti)。實施(shi)(shi)例(li)(li)4在(zai)實施(shi)(shi)例(li)(li)1的(de)(de)(de)基(ji)礎上提供(gong)的(de)(de)(de)一(yi)(yi)種(zhong)高(gao)(gao)原(yuan)性(xing)(xing)人(ren)類疾病(bing)模(mo)(mo)型制(zhi)備(bei)(bei)(bei)環(huan)境模(mo)(mo)擬系(xi)(xi)(xi)統(tong)(tong)(tong)。（arteriosclerosis，AS）是一(yi)(yi)種(zhong)緩(huan)慢進行(xing)(xing)性(xing)(xing)疾病(bing)，嚴(yan)重影響人(ren)類健康。

e：不同光(guang)(guang)(guang)強(qiang)下(xia)gm20541基因(yin)(yin)(yin)(yin)(yin)敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)小(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)暗適(shi)應(ying)(ying)(ying)(ying)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)電(dian)圖(tu)(tu)b波統(tong)計(ji)；scotopicamplitude：暗光(guang)(guang)(guang)測定峰(feng)值；flashintensity：閃(shan)光(guang)(guang)(guang)強(qiang)度(du)(du)；a-wave：a波；b-wave：b波。圖(tu)(tu)6：光(guang)(guang)(guang)適(shi)應(ying)(ying)(ying)(ying)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)電(dian)圖(tu)(tu)(erg)檢測結果；圖(tu)(tu)中：a-b：不同光(guang)(guang)(guang)強(qiang)下(xia)gm20541基因(yin)(yin)(yin)(yin)(yin)敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)小(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)光(guang)(guang)(guang)適(shi)應(ying)(ying)(ying)(ying)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)電(dian)圖(tu)(tu)軌跡圖(tu)(tu)；c：20cd·s/m2光(guang)(guang)(guang)強(qiang)下(xia)gm20541基因(yin)(yin)(yin)(yin)(yin)敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)小(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)光(guang)(guang)(guang)適(shi)應(ying)(ying)(ying)(ying)閃(shan)爍(flicker)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)電(dian)圖(tu)(tu)軌跡圖(tu)(tu)；d：不同光(guang)(guang)(guang)強(qiang)下(xia)gm20541基因(yin)(yin)(yin)(yin)(yin)敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)小(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)光(guang)(guang)(guang)適(shi)應(ying)(ying)(ying)(ying)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)電(dian)圖(tu)(tu)a波統(tong)計(ji)；e：不同光(guang)(guang)(guang)強(qiang)下(xia)gm20541基因(yin)(yin)(yin)(yin)(yin)敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)小(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)光(guang)(guang)(guang)適(shi)應(ying)(ying)(ying)(ying)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)電(dian)圖(tu)(tu)b波統(tong)計(ji)；f：20cd·s/m2光(guang)(guang)(guang)強(qiang)下(xia)gm20541基因(yin)(yin)(yin)(yin)(yin)敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)小(xiao)鼠(shu)(shu)(shu)(shu)的(de)(de)(de)光(guang)(guang)(guang)適(shi)應(ying)(ying)(ying)(ying)閃(shan)爍(flicker)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)電(dian)圖(tu)(tu)統(tong)計(ji)。sixko或cko表示gm20541基因(yin)(yin)(yin)(yin)(yin)敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)純合(he)(he)子小(xiao)鼠(shu)(shu)(shu)(shu)；ctr是(shi)指野(ye)生型(xing)；het是(shi)指雜合(he)(he)子。圖(tu)(tu)7：視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)前(qian)體(ti)細胞(bao)特(te)異敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)gm20541基因(yin)(yin)(yin)(yin)(yin)小(xiao)鼠(shu)(shu)(shu)(shu)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)切(qie)片免疫組(zu)化染色結果；小(xiao)鼠(shu)(shu)(shu)(shu)年齡：4個月；os：outer-segment(外(wai)節(jie)(jie))；is：inner-segment(內節(jie)(jie))；onl：outernuclearlayer(外(wai)核(he)(he)層(ceng))；inl：innernuclearlayer(內核(he)(he)層(ceng))；圖(tu)(tu)中：a：視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)前(qian)體(ti)細胞(bao)特(te)異敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)gm20541基因(yin)(yin)(yin)(yin)(yin)小(xiao)鼠(shu)(shu)(shu)(shu)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)石蠟切(qie)片的(de)(de)(de)h&e染色結果，外(wai)核(he)(he)層(ceng)及內核(he)(he)層(ceng)均變薄(bo)；b：對不同部位的(de)(de)(de)gm20541敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)鼠(shu)(shu)(shu)(shu)視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)外(wai)核(he)(he)層(ceng)厚度(du)(du)統(tong)計(ji)。圖(tu)(tu)8：視(shi)(shi)網(wang)(wang)(wang)(wang)膜(mo)前(qian)體(ti)細胞(bao)特(te)異敲(qiao)(qiao)除(chu)(chu)(chu)(chu)(chu)gm20541基因(yin)(yin)(yin)(yin)(yin)小(xiao)鼠(shu)(shu)(shu)(shu)ihc染色結果。好(hao)的(de)(de)(de)動(dong)物(wu)模(mo)型(xing)能夠(gou)較好(hao)地模(mo)擬疾病狀態，為的(de)(de)(de)研究提供(gong)可能。云南C57動(dong)物(wu)模(mo)型(xing)手術

IgA腎病小(xiao)鼠模(mo)型IgA 腎病是常見(jian)的原發性腎小(xiao)球(qiu)疾病,約占原發性腎小(xiao)球(qiu)疾病的 1/3。云(yun)南C57動(dong)物模(mo)型手術

靜置25分(fen)鐘(zhong)(zhong)后(hou)(hou)把(ba)(ba)酒(jiu)精倒干，用(yong)吸水紙吸出(chu)(chu)多(duo)余的(de)酒(jiu)精，然(ran)(ran)后(hou)(hou)配壓縮(suo)膠(jiao)，同樣的(de)操作，關鍵是梳(shu)子要(yao)插得快，要(yao)小(xiao)(xiao)心(xin)梳(shu)子下(xia)產生氣泡(pao)，然(ran)(ran)后(hou)(hou)靜置30分(fen)鐘(zhong)(zhong)。如果是當(dang)天(tian)跑膠(jiao)，我會(hui)(hui)(hui)等(deng)上(shang)(shang)(shang)層膠(jiao)凝(ning)2個(ge)小(xiao)(xiao)時再用(yong)，但要(yao)注(zhu)意防干燥縮(suo)水，可以在一(yi)個(ge)小(xiao)(xiao)時的(de)時候沿(yan)著梳(shu)子上(shang)(shang)(shang)緣加點(dian)電(dian)(dian)泳液(ye)。所以我一(yi)般提前一(yi)晚制膠(jiao)，泡(pao)于(yu)(yu)純水或(huo)者電(dian)(dian)泳液(ye)里置于(yu)(yu)4度冰(bing)箱暫存。三、蛋白(bai)電(dian)(dian)泳1、上(shang)(shang)(shang)樣前準(zhun)(zhun)備(bei)把(ba)(ba)膠(jiao)組(zu)裝到電(dian)(dian)泳芯上(shang)(shang)(shang)，注(zhu)意密閉(bi)性(否則(ze)漏液(ye))，如果內槽(cao)漏液(ye)就不(bu)是勻強電(dian)(dian)場了，條(tiao)帶可能就不(bu)是一(yi)條(tiao)直線。然(ran)(ran)后(hou)(hou)內槽(cao)倒滿(man)電(dian)(dian)泳液(ye)，拔(ba)(ba)梳(shu)子，這一(yi)步要(yao)小(xiao)(xiao)心(xin)，梳(shu)子要(yao)兩邊一(yi)起緩緩往上(shang)(shang)(shang)拔(ba)(ba)出(chu)(chu)，然(ran)(ran)后(hou)(hou)觀(guan)察泳道內有無脫落的(de)膠(jiao)粒或(huo)者膠(jiao)絲，有的(de)話用(yong)1毫升注(zhu)射器(qi)(qi)吸出(chu)(chu)。然(ran)(ran)后(hou)(hou)從冰(bing)箱取(qu)出(chu)(chu)蛋白(bai)樣品(pin)，解凍(dong)。準(zhun)(zhun)備(bei)振(zhen)蕩(dang)器(qi)(qi)。2、上(shang)(shang)(shang)樣和電(dian)(dian)泳注(zhu)意，上(shang)(shang)(shang)樣后(hou)(hou)蛋白(bai)會(hui)(hui)(hui)開(kai)始慢(man)(man)慢(man)(man)在膠(jiao)中彌散(san)，所以上(shang)(shang)(shang)樣越快越好(hao)(hao)。我習慣先上(shang)(shang)(shang)蛋白(bai)Marker,再上(shang)(shang)(shang)蛋白(bai)樣品(pin)，蛋白(bai)上(shang)(shang)(shang)樣前確保(bao)樣品(pin)完全解凍(dong)和充分(fen)振(zhen)蕩(dang)(推薦使(shi)用(yong)振(zhen)蕩(dang)器(qi)(qi)振(zhen)蕩(dang))，吸的(de)時候沒(mei)有拉(la)絲即可，建議上(shang)(shang)(shang)樣分(fen)鐘(zhong)(zhong)把(ba)(ba)樣品(pin)從冰(bing)上(shang)(shang)(shang)取(qu)出(chu)(chu)來，不(bu)然(ran)(ran)樣品(pin)中SDS可能會(hui)(hui)(hui)結(jie)晶析出(chu)(chu)，從而影響(xiang)電(dian)(dian)泳效果。上(shang)(shang)(shang)層膠(jiao)80V25分(fen)鐘(zhong)(zhong)，下(xia)層膠(jiao)120V65分(fen)鐘(zhong)(zhong)。四、轉(zhuan)膜1、轉(zhuan)膜前準(zhun)(zhun)備(bei)我會(hui)(hui)(hui)在電(dian)(dian)泳結(jie)束0分(fen)鐘(zhong)(zhong)準(zhun)(zhun)備(bei)，把(ba)(ba)轉(zhuan)膜液(ye)配好(hao)(hao)置于(yu)(yu)4度冰(bing)箱預冷，然(ran)(ran)后(hou)(hou)裁膜，準(zhun)(zhun)備(bei)轉(zhuan)膜裝置。云南C57動(dong)物模型手(shou)術

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