1.首要(yao)(yao)原則：細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)不(bu)重(zhong)要(yao)(yao)情況下(xia)(xia)立即丟棄，培(pei)(pei)養(yang)箱(xiang)滅(mie)菌，所用培(pei)(pei)養(yang)基(ji)(ji)也都要(yao)(yao)丟棄，器械(xie)等重(zhong)新(xin)滅(mie)菌或拆用新(xin)的(de)。2.細(xi)(xi)(xi)(xi)菌污(wu)染(ran)(ran)(ran)一(yi)般(ban)都救不(bu)回(hui)來(lai)了(le)，發現的(de)時(shi)候(hou)培(pei)(pei)養(yang)基(ji)(ji)一(yi)般(ban)都很渾濁且細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)都死了(le)3.污(wu)染(ran)(ran)(ran)且細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)很重(zhong)要(yao)(yao)時(shi)：遇到(dao)念球(qiu)(qiu)(qiu)菌污(wu)染(ran)(ran)(ran)，且細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)為基(ji)(ji)因改(gai)造細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，非常重(zhong)要(yao)(yao)。如231貼壁(bi)乳腺細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)，發現細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)周(zhou)圍出現很小的(de)串珠(zhu)透(tou)亮圓(yuan)點(dian)，非常像念球(qiu)(qiu)(qiu)菌污(wu)染(ran)(ran)(ran)，此(ci)時(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)狀態尚可(ke)(ke)(ke)，且污(wu)染(ran)(ran)(ran)少。處(chu)理(li)如下(xia)(xia)：用預(yu)熱或室溫PBS清洗(xi)3次(ci)，可(ke)(ke)(ke)適當振搖，將污(wu)染(ran)(ran)(ran)沖洗(xi)下(xia)(xia)來(lai)。隨后(hou)(hou)加入(ru)10-20%雙(shuang)抗到(dao)培(pei)(pei)養(yang)瓶(ping)，置于37度培(pei)(pei)養(yang)箱(xiang)1h，之后(hou)(hou)再用PBS清洗(xi)三遍，直至(zhi)視野(ye)下(xia)(xia)無可(ke)(ke)(ke)見污(wu)染(ran)(ran)(ran)。此(ci)時(shi)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)也被沖下(xia)(xia)大部分，因此(ci)此(ci)方法只適用在細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)貼壁(bi)強，狀態好，密度高時(shi)使(shi)用。之后(hou)(hou)每天再更(geng)換培(pei)(pei)養(yang)基(ji)(ji)，每次(ci)用PBS沖洗(xi)2遍。過幾天細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)狀態尚可(ke)(ke)(ke)時(shi)，消化(hua)離心(xin)時(shi)用500r，3min，去掉(diao)上清，重(zhong)復3次(ci)。這(zhe)個(ge)方法是(shi)(shi)根據文獻(xian)可(ke)(ke)(ke)利(li)用念球(qiu)(qiu)(qiu)菌和(he)細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)體積(ji)重(zhong)量差(cha)異(yi)實(shi)(shi)現分離。基(ji)(ji)本(ben)上這(zhe)一(yi)步做完以后(hou)(hou)，污(wu)染(ran)(ran)(ran)就基(ji)(ji)本(ben)了(le)，接下(xia)(xia)來(lai)就注意多(duo)觀察(cha)，勤換液就行。細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)增殖(zhi)與細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凋(diao)亡(wang)、細(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)周(zhou)期(qi)等是(shi)(shi)研究的(de)重(zhong)要(yao)(yao)表型，是(shi)(shi)分子生物學(xue)和(he)藥(yao)理(li)學(xue)研究解決的(de)問題之一(yi)。廣(guang)西(xi)組織科研技術服務實(shi)(shi)驗室

轉錄組測(ce)序結果(guo)及TCGA數(shu)據(ju)庫(ku)分(fen)(fen)(fen)析）圖5RNA-Seq和m6A-seq聯合鑒(jian)定SOCS2是介導的(de)(de)m6A修飾(shi)的(de)(de)下游靶基(ji)(ji)因PLoSOne2015,在(zai)許多不同種(zhong)類的(de)(de)RNA中，都已觀(guan)察(cha)到N6-腺苷(m6A)的(de)(de)甲(jia)(jia)基(ji)(ji)化(hua)，但其(qi)在(zai)microRNAs中還沒有(you)被研究。研究者在(zai)FTO1C1,FTO2D4和FTO3C3細胞系中，通(tong)(tong)過敲(qiao)除(chu)m6A甲(jia)(jia)基(ji)(ji)轉移酶FTO篩選到表達(da)差異的(de)(de)microRNA,說明miRNA受m6A甲(jia)(jia)基(ji)(ji)化(hua)的(de)(de)調控。進一(yi)(yi)步通(tong)(tong)過MeRIP-Seq發(fa)現相當一(yi)(yi)部(bu)分(fen)(fen)(fen)的(de)(de)microRNA具有(you)m6A修飾(shi)。通(tong)(tong)過motif分(fen)(fen)(fen)析，他們發(fa)現了區分(fen)(fen)(fen)甲(jia)(jia)基(ji)(ji)化(hua)和非甲(jia)(jia)基(ji)(ji)化(hua)microRNA的(de)(de)一(yi)(yi)致(zhi)序列。該(gai)文章所述的(de)(de)表觀(guan)遺(yi)傳(chuan)修飾(shi)在(zai)基(ji)(ji)因表達(da)的(de)(de)轉錄調控的(de)(de)復雜性上增加了一(yi)(yi)個(ge)新的(de)(de)層次。圖FTO敲(qiao)除(chu)對(dui)甲(jia)(jia)基(ji)(ji)化(hua)的(de)(de)miRNAs的(de)(de)穩定狀態的(de)(de)影響。參(can)考(kao)文獻Y,DominissiniD,RechaviG,HeC:Geneexpressionregulationmediatedthroughreversiblem(6)(5):'(1):(12):(6):(1):(1):(uridine)(41):(6)(7540):(1):(7481):(4):(6)A-LAIC-seqrevealsthecensusandcomplexityofthem(6)(8):'UTRm(6)(4):(7544):(6)(6):(5):(7667):(2):"">panstyle="color:#f5c81c;">xiainducesthebreastcancerstemcellphenotypebyHIF-dependentandALKBH5-mediatedm(6)(14):"">panstyle="color:#f5c81c;">(40):(6)(3):(1):(1):(4):(11):。寧夏動物科研技術(shu)服務分(fen)(fen)(fen)離干貨分(fen)(fen)(fen)享｜選對(dui)實驗室常用培養(yang)基(ji)(ji)DMEM和1640。

應退(tui)回純酒精(jing)(jing)中(zhong)(zhong)重新脫水(shui)，然(ran)后再透(tou)明(ming)，否則切片難以(yi)鏡(jing)檢(jian)。（4）二(er)甲苯應盡量(liang)保(bao)持無(wu)水(shui)，應經(jing)常更換，或(huo)用紗(sha)布包無(wu)水(shui)硫酸銅放(fang)入(ru)染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)缸內(nei)吸收水(shui)分。5.透(tou)蠟(la)注(zhu)意(yi)(yi)(yi)事項(xiang)（1）透(tou)蠟(la)的(de)目的(de)是除(chu)去組(zu)織(zhi)(zhi)中(zhong)(zhong)的(de)透(tou)明(ming)劑（如(ru)二(er)甲苯等(deng)），使石蠟(la)滲透(tou)到(dao)組(zu)織(zhi)(zhi)內(nei)部(bu)達到(dao)飽和程(cheng)(cheng)度以(yi)便包埋。透(tou)蠟(la)時(shi)(shi)間(jian)根據(ju)組(zu)織(zhi)(zhi)大小而定(ding)。（2）盡量(liang)保(bao)持在較低(di)溫度中(zhong)(zhong)進行，以(yi)石蠟(la)不(bu)(bu)凝固為度。（3）透(tou)蠟(la)溫度要(yao)恒定(ding)，不(bu)(bu)可(ke)忽高(gao)忽低(di)。（4）操作要(yao)迅速，力求(qiu)在短的(de)時(shi)(shi)間(jian)內(nei)完成石蠟(la)透(tou)入(ru)過(guo)程(cheng)(cheng)，以(yi)免引起組(zu)織(zhi)(zhi)變(bian)硬、變(bian)脆、收縮(suo)等(deng)。（5）注(zhu)意(yi)(yi)(yi)溫度不(bu)(bu)要(yao)過(guo)高(gao)，以(yi)免組(zu)織(zhi)(zhi)發脆。6.染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)水(shui)洗注(zhu)意(yi)(yi)(yi)事項(xiang)（1）染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)原理：伊紅主(zhu)要(yao)染(ran)(ran)(ran)(ran)(ran)(ran)細(xi)胞(bao)質(zhi)(zhi)，著色(se)(se)濃(nong)淡應與蘇木精(jing)(jing)染(ran)(ran)(ran)(ran)(ran)(ran)細(xi)胞(bao)核的(de)濃(nong)淡相配(pei)合，如(ru)果(guo)細(xi)胞(bao)核染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)較濃(nong)，細(xi)胞(bao)質(zhi)(zhi)也應濃(nong)染(ran)(ran)(ran)(ran)(ran)(ran)，以(yi)獲得鮮明(ming)的(de)對比。（2）染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)時(shi)(shi)間(jian)應根據(ju)染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)劑的(de)成熟程(cheng)(cheng)度及室溫高(gao)低(di)，適當縮(suo)短或(huo)延長。室溫高(gao)時(shi)(shi)促進染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)，染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)時(shi)(shi)間(jian)可(ke)短些(xie)，否則可(ke)適當延長時(shi)(shi)間(jian)，冬季室溫低(di)時(shi)(shi)可(ke)放(fang)入(ru)恒溫箱(xiang)中(zhong)(zhong)染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)。（3）注(zhu)意(yi)(yi)(yi)流水(shui)不(bu)(bu)能過(guo)大，以(yi)防切片脫落。（4）如(ru)果(guo)細(xi)胞(bao)核染(ran)(ran)(ran)(ran)(ran)(ran)色(se)(se)較淺(qian)，細(xi)胞(bao)質(zhi)(zhi)也應淡染(ran)(ran)(ran)(ran)(ran)(ran)。可(ke)在伊紅乙(yi)醇液中(zhong)(zhong)滴加(jia)數滴冰醋酸助染(ran)(ran)(ran)(ran)(ran)(ran)，促使細(xi)胞(bao)質(zhi)(zhi)容易著色(se)(se)，并且(qie)經(jing)乙(yi)醇脫水(shui)時(shi)(shi)不(bu)(bu)易褪色(se)(se)。

啟醫療(liao)，個體(ti)化(hua)(hua)用(yong)(yong)藥貝克曼庫(ku)(ku)爾特(te)細(xi)胞專(zhuan)題(ti)--助力病(bing)毒載體(ti)純化(hua)(hua)、細(xi)胞特(te)性分(fen)(fen)析產品(pin)(pin)庫(ku)(ku)儀(yi)器設備(bei)耗材試(shi)劑抗體(ti)技術(shu)服務生(sheng)(sheng)物(wu)(wu)(wu)(wu)芯(xin)(xin)片芯(xin)(xin)片掃描儀(yi)|芯(xin)(xin)片點(dian)樣儀(yi)|生(sheng)(sheng)物(wu)(wu)(wu)(wu)芯(xin)(xin)片系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|生(sheng)(sheng)物(wu)(wu)(wu)(wu)芯(xin)(xin)片|其(qi)(qi)它(ta)(ta)測(ce)(ce)(ce)讀(du)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)洗板機|多功(gong)能(neng)篩選(xuan)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|大型分(fen)(fen)析系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|化(hua)(hua)學發光(guang)檢(jian)測(ce)(ce)(ce)儀(yi)|分(fen)(fen)光(guang)光(guang)度(du)計|其(qi)(qi)它(ta)(ta)|光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)原子吸收光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|可見光(guang)光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|熒光(guang)光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|紅外(wai)光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|近(jin)紅外(wai)光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|LIBS光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|拉(la)曼光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|紫外(wai)/可見/近(jin)紅外(wai)光(guang)譜(pu)(pu)(pu)(pu)(pu)(pu)儀(yi)|其(qi)(qi)它(ta)(ta)分(fen)(fen)子生(sheng)(sheng)物(wu)(wu)(wu)(wu)實(shi)驗儀(yi)器電泳設備(bei)|紫外(wai)設備(bei)|普通PCR儀(yi)|定量PCR儀(yi)|數字(zi)PCR儀(yi)|DNA/有機/多肽合成|轉(zhuan)基(ji)(ji)因儀(yi)|其(qi)(qi)它(ta)(ta)顯微(wei)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)倒置顯微(wei)鏡(jing)|實(shi)體(ti)顯微(wei)鏡(jing)|生(sheng)(sheng)物(wu)(wu)(wu)(wu)顯微(wei)鏡(jing)|電子顯微(wei)鏡(jing)其(qi)(qi)它(ta)(ta)顯微(wei)鏡(jing)|顯微(wei)操縱|附件和(he)濾光(guang)片|其(qi)(qi)它(ta)(ta)色(se)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)液(ye)(ye)相色(se)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|制(zhi)備(bei)液(ye)(ye)相色(se)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|毛細(xi)管LC系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)氣相色(se)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|離子色(se)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|色(se)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)檢(jian)測(ce)(ce)(ce)器樣品(pin)(pin)管理(li)工具|色(se)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)附件質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)飛行質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)|四極桿質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)|離子阱質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)|等離子質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)|毛細(xi)管電泳/質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)聯(lian)用(yong)(yong)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|雜交(jiao)質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)|質(zhi)(zhi)(zhi)(zhi)譜(pu)(pu)(pu)(pu)(pu)(pu)標準(zhun)品(pin)(pin)|其(qi)(qi)它(ta)(ta)實(shi)驗室(shi)自(zi)動(dong)化(hua)(hua)氨(an)基(ji)(ji)酸(suan)分(fen)(fen)析系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|蛋白純化(hua)(hua)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|蛋白質(zhi)(zhi)(zhi)(zhi)翻譯系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|全自(zi)動(dong)分(fen)(fen)液(ye)(ye)系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|核酸(suan)提取純化(hua)(hua)全自(zi)動(dong)血液(ye)(ye)采樣系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|基(ji)(ji)因組(zu)/蛋白組(zu)設備(bei)DNA測(ce)(ce)(ce)序(xu)儀(yi)|全基(ji)(ji)因組(zu)測(ce)(ce)(ce)序(xu)儀(yi)|基(ji)(ji)因分(fen)(fen)型系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|雙向(xiang)電泳系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|蛋白質(zhi)(zhi)(zhi)(zhi)純化(hua)(hua)|蛋白質(zhi)(zhi)(zhi)(zhi)斑點(dian)切取系(xi)(xi)(xi)(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)(tong)|其(qi)(qi)它(ta)(ta)神經生(sheng)(sheng)物(wu)(wu)(wu)(wu)學儀(yi)器動(dong)物(wu)(wu)(wu)(wu)功(gong)能(neng)檢(jian)測(ce)(ce)(ce)設備(bei)|動(dong)物(wu)(wu)(wu)(wu)處理(li)設備(bei)|。隨著科(ke)技的不(bu)斷進(jin)步，科(ke)研人(ren)員將(jiang)能(neng)夠開發出更為精確(que)、實(shi)用(yong)(yong)的動(dong)物(wu)(wu)(wu)(wu)模(mo)型，更好(hao)地為人(ren)類健(jian)康保駕護(hu)航。

動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)在(zai)(zai)科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)中(zhong)有(you)(you)著普遍(bian)的(de)應(ying)用(yong)。首(shou)先，它們(men)可(ke)以(yi)(yi)(yi)幫助科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)人(ren)(ren)員(yuan)(yuan)(yuan)深入理解的(de)共(gong)同性(xing)，即不同物(wu)(wu)(wu)(wu)種之間存(cun)(cun)在(zai)(zai)的(de)共(gong)有(you)(you)理變化過(guo)(guo)程(cheng)。通(tong)過(guo)(guo)對動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)的(de)研(yan)(yan)(yan)(yan)究(jiu)，科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)人(ren)(ren)員(yuan)(yuan)(yuan)可(ke)以(yi)(yi)(yi)更(geng)清楚地了解的(de)發(fa)(fa)展過(guo)(guo)程(cheng)和機(ji)制(zhi)，為(wei)人(ren)(ren)類的(de)檢查(cha)提(ti)(ti)供(gong)(gong)理論依(yi)據。其次，動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)還為(wei)新研(yan)(yan)(yan)(yan)發(fa)(fa)和苗(miao)測試(shi)提(ti)(ti)供(gong)(gong)了的(de)平臺。在(zai)(zai)研(yan)(yan)(yan)(yan)發(fa)(fa)過(guo)(guo)程(cheng)中(zhong)，科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)人(ren)(ren)員(yuan)(yuan)(yuan)可(ke)以(yi)(yi)(yi)通(tong)過(guo)(guo)對動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)進(jin)行(xing)處理，觀察其和副作用(yong)，為(wei)新的(de)臨(lin)床試(shi)驗(yan)提(ti)(ti)供(gong)(gong)依(yi)據。而(er)在(zai)(zai)苗(miao)測試(shi)中(zhong)，動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)則可(ke)以(yi)(yi)(yi)用(yong)來評估苗(miao)的(de)性(xing)和安全性(xing)。此(ci)外，動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)還為(wei)科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)人(ren)(ren)員(yuan)(yuan)(yuan)提(ti)(ti)供(gong)(gong)了研(yan)(yan)(yan)(yan)究(jiu)人(ren)(ren)類的(de)跨(kua)學科(ke)(ke)(ke)方法。例如，通(tong)過(guo)(guo)比較(jiao)人(ren)(ren)類和動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)的(de)基因組(zu)學、蛋(dan)白質組(zu)學等(deng)數據，可(ke)以(yi)(yi)(yi)發(fa)(fa)現與發(fa)(fa)生(sheng)相(xiang)關的(de)關鍵基因和蛋(dan)白質，從(cong)(cong)而(er)為(wei)的(de)和檢查(cha)提(ti)(ti)供(gong)(gong)新的(de)思(si)路(lu)。雖然動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)在(zai)(zai)科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)中(zhong)發(fa)(fa)揮了巨大(da)的(de)作用(yong)，但(dan)也(ye)存(cun)(cun)在(zai)(zai)一些挑(tiao)戰。首(shou)先，由(you)于(yu)物(wu)(wu)(wu)(wu)種差異的(de)存(cun)(cun)在(zai)(zai)，動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)的(de)表現與人(ren)(ren)類可(ke)能存(cun)(cun)在(zai)(zai)差異，因此(ci)需(xu)要謹慎使用(yong)。此(ci)外，動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)的(de)倫(lun)(lun)理問題(ti)也(ye)不容(rong)忽視，科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)人(ren)(ren)員(yuan)(yuan)(yuan)需(xu)要在(zai)(zai)符合倫(lun)(lun)理規定的(de)前提(ti)(ti)下進(jin)行(xing)相(xiang)關研(yan)(yan)(yan)(yan)究(jiu)。盡管存(cun)(cun)在(zai)(zai)挑(tiao)戰，動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)的(de)發(fa)(fa)展前景(jing)仍然值得期待。隨著科(ke)(ke)(ke)技的(de)不斷進(jin)步，科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)人(ren)(ren)員(yuan)(yuan)(yuan)將(jiang)能夠(gou)開發(fa)(fa)出更(geng)為(wei)精(jing)確、實(shi)用(yong)的(de)動(dong)(dong)物(wu)(wu)(wu)(wu)模(mo)(mo)(mo)(mo)型(xing)。可(ke)以(yi)(yi)(yi)發(fa)(fa)現與疾病(bing)(bing)發(fa)(fa)生(sheng)相(xiang)關的(de)關鍵基因和蛋(dan)白質，從(cong)(cong)而(er)為(wei)疾病(bing)(bing)的(de)預防(fang)和檢查(cha)提(ti)(ti)供(gong)(gong)新的(de)思(si)路(lu)。黑龍江科(ke)(ke)(ke)研(yan)(yan)(yan)(yan)技術服務分離

動物模(mo)型的(de)表現與人類疾病(bing)可能(neng)存在(zai)差異，因(yin)此需要(yao)謹慎使(shi)用。廣西組(zu)織(zhi)科研技術服務(wu)實驗(yan)室

首先(xian)，預實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)必須要(yao)(yao)當做正式實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)來(lai)對待（態度要(yao)(yao)放端正，這是必須的(de)(de)）。預實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)的(de)(de)每一步都需(xu)要(yao)(yao)詳細規劃(hua)，多(duo)方(fang)籌謀。不論是實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)的(de)(de)選擇，還是檢測試(shi)劑的(de)(de)購(gou)(gou)買(mai)(mai)，都盡量(liang)和(he)正式實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)統一標準。建議(yi)大家先(xian)把(ba)所(suo)有(you)(you)試(shi)劑和(he)購(gou)(gou)買(mai)(mai)完畢后，再去購(gou)(gou)買(mai)(mai)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)。因(yin)(yin)為(wei)動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)會(hui)長胖(pang)(pang)，長胖(pang)(pang)后給劑量(liang)就要(yao)(yao)增加(jia)，不僅(jin)多(duo)花錢(qian)(qian)，并且還容易影響實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)效果(guo)。筆(bi)者曾經提(ti)前將實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)買(mai)(mai)回(hui)，但因(yin)(yin)為(wei)特(te)殊原因(yin)(yin)沒(mei)能(neng)購(gou)(gou)買(mai)(mai)到(dao)造(zao)模(mo)，終(zhong)只能(neng)忍痛割愛將動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)贈送他人，白(bai)白(bai)虧了一筆(bi)血汗錢(qian)(qian)（那批老鼠在我這兒白(bai)吃白(bai)喝(he)長得(de)太胖(pang)(pang)，沒(mei)辦法(fa)用作造(zao)模(mo)）。動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)及試(shi)劑購(gou)(gou)買(mai)(mai)首先(xian)需(xu)要(yao)(yao)考慮：實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)品種、體(ti)重(zhong)、性別、周齡（通(tong)常根據既往文獻(xian)報道可(ke)以獲(huo)得(de)答(da)案）、數量(liang)（需(xu)要(yao)(yao)考慮到(dao)實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)有(you)(you)一定動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)死亡率或者動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)本身會(hui)打架互毆，因(yin)(yin)此需(xu)要(yao)(yao)提(ti)前購(gou)(gou)買(mai)(mai)足(zu)夠(gou)的(de)(de)動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)）。動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)購(gou)(gou)買(mai)(mai)的(de)(de)途徑、安(an)置的(de)(de)地(di)點(dian)，飲食管(guan)理（一般實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)室(shi)會(hui)有(you)(you)動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)房，也可(ke)以從其他地(di)方(fang)購(gou)(gou)買(mai)(mai)），動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)免證(zheng)明(ming)、倫理證(zheng)明(ming)需(xu)要(yao)(yao)提(ti)前準備好(hao)。實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)相關的(de)(de)試(shi)劑和(he)：比如造(zao)模(mo)和(he)，動(dong)(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)(wu)干預所(suo)需(xu)，陽(yang)性選擇及購(gou)(gou)買(mai)(mai)（有(you)(you)些(xie)購(gou)(gou)買(mai)(mai)很困(kun)難，必須通(tong)過特(te)殊程序才(cai)能(neng)買(mai)(mai)到(dao)）。如果(guo)涉及到(dao)有(you)(you)創操作，要(yao)(yao)提(ti)前準備好(hao)（建議(yi)提(ti)前購(gou)(gou)買(mai)(mai)），手術(shu)。需(xu)要(yao)(yao)了解(jie)自(zi)身實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)平臺能(neng)完成哪些(xie)技術(shu)。廣西組織科研技術(shu)服務實(shi)(shi)(shi)驗(yan)(yan)(yan)(yan)(yan)室(shi)

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