染(ran)色(se)的(de)(de)(de)一般原則(ze)：因檢測(ce)(ce)細(xi)胞的(de)(de)(de)類型、凋亡誘導劑(ji)種(zhong)類、使(shi)用(yong)(yong)的(de)(de)(de)檢測(ce)(ce)儀器(qi)不(bu)同，因而流式檢測(ce)(ce)的(de)(de)(de)熒(ying)光(guang)(guang)補償也不(bu)同，因此(ci)建(jian)議(yi)每次檢測(ce)(ce)均需(xu)使(shi)用(yong)(yong)凋亡誘導處(chu)理的(de)(de)(de)細(xi)胞做單(dan)陽對照，進行熒(ying)光(guang)(guang)補償的(de)(de)(de)調(diao)節。標記抗體常用(yong)(yong)熒(ying)光(guang)(guang)素(su)FITC，EGFP激(ji)發光(guang)(guang)：488nm發射光(guang)(guang)：525nm;使(shi)用(yong)(yong)面(mian)較(jiao)廣(guang)，價格便宜。PE激(ji)發光(guang)(guang)488nm發射光(guang)(guang)575nm，此(ci)熒(ying)光(guang)(guang)的(de)(de)(de)激(ji)發效率較(jiao)佳，故此(ci)熒(ying)光(guang)(guang)得(de)到的(de)(de)(de)信號較(jiao)強；檢測(ce)(ce)某些(xie)弱表達的(de)(de)(de)抗原，推薦使(shi)用(yong)(yong)此(ci)熒(ying)光(guang)(guang)素(su)。價格較(jiao)FITC昂(ang)貴。APC激(ji)發光(guang)(guang)633nm，發射光(guang)(guang)660nm，在配有(you)雙激(ji)光(guang)(guang)的(de)(de)(de)流式細(xi)胞儀上，此(ci)熒(ying)光(guang)(guang)染(ran)料可與7-AAD或(huo)PI共同使(shi)用(yong)(yong)來避開(kai)自帶綠色(se)熒(ying)光(guang)(guang)的(de)(de)(de)樣本。流式細(xi)胞儀相關試劑(ji)盒。為了您的(de)(de)(de)安全和健康，請穿實驗服并戴一次性手(shou)套(tao)操作(zuo)。天(tian)津(jin)如何(he)使(shi)用(yong)(yong)糖原染(ran)色(se)試劑(ji)盒量大從優

糖原染色正常值:正常情況下，紅(hong)細(xi)(xi)(xi)(xi)胞(bao)系(xi)統的(de)(de)原、幼(you)紅(hong)細(xi)(xi)(xi)(xi)胞(bao)和成(cheng)熟紅(hong)細(xi)(xi)(xi)(xi)胞(bao);粒細(xi)(xi)(xi)(xi)胞(bao)系(xi)統的(de)(de)原粒細(xi)(xi)(xi)(xi)胞(bao)、原單核(he)細(xi)(xi)(xi)(xi)胞(bao)和大多(duo)數淋(lin)巴(ba)細(xi)(xi)(xi)(xi)胞(bao)為陰(yin)(yin)性(xing)反(fan)(fan)應(ying)(ying)(ying)。自早幼(you)粒階段以后的(de)(de)粒細(xi)(xi)(xi)(xi)胞(bao)和幼(you)單核(he)細(xi)(xi)(xi)(xi)胞(bao)可呈(cheng)(cheng)弱(ruo)(ruo)陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying)。糖原染色臨床意義:(1)急性(xing)淋(lin)巴(ba)細(xi)(xi)(xi)(xi)胞(bao)白(bai)血(xue)病、淋(lin)巴(ba)組(zu)織惡性(xing)增(zeng)生(sheng)(sheng)性(xing)疾病、紅(hong)白(bai)血(xue)病、戈謝病的(de)(de)原始細(xi)(xi)(xi)(xi)胞(bao)呈(cheng)(cheng)強陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying)或(huo)陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying);缺鐵性(xing)貧血(xue)、珠蛋白(bai)生(sheng)(sheng)成(cheng)障礙、骨髓增(zeng)生(sheng)(sheng)異常綜合征(zheng)亦可呈(cheng)(cheng)陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying);急性(xing)粒細(xi)(xi)(xi)(xi)胞(bao)白(bai)血(xue)病、急性(xing)單核(he)細(xi)(xi)(xi)(xi)胞(bao)白(bai)血(xue)病、良性(xing)淋(lin)巴(ba)細(xi)(xi)(xi)(xi)胞(bao)增(zeng)多(duo)癥、尼(ni)曼-皮克細(xi)(xi)(xi)(xi)胞(bao)呈(cheng)(cheng)陰(yin)(yin)性(xing)反(fan)(fan)應(ying)(ying)(ying)或(huo)弱(ruo)(ruo)陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying)。巨(ju)幼(you)細(xi)(xi)(xi)(xi)胞(bao)性(xing)貧血(xue)、溶血(xue)性(xing)貧血(xue)、再生(sheng)(sheng)障礙性(xing)貧血(xue)等，幼(you)紅(hong)細(xi)(xi)(xi)(xi)胞(bao)為陰(yin)(yin)性(xing)反(fan)(fan)應(ying)(ying)(ying)。偶有個(ge)別(bie)幼(you)紅(hong)細(xi)(xi)(xi)(xi)胞(bao)呈(cheng)(cheng)陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying)。(2)幫(bang)助鑒別(bie)不典型巨(ju)核(he)細(xi)(xi)(xi)(xi)胞(bao)和霍(huo)奇(qi)金(jin)細(xi)(xi)(xi)(xi)胞(bao)，巨(ju)核(he)細(xi)(xi)(xi)(xi)胞(bao)呈(cheng)(cheng)強陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying);霍(huo)奇(qi)金(jin)細(xi)(xi)(xi)(xi)胞(bao)呈(cheng)(cheng)弱(ruo)(ruo)陽(yang)(yang)(yang)性(xing)或(huo)陰(yin)(yin)性(xing)反(fan)(fan)應(ying)(ying)(ying)。(3)幫(bang)助鑒別(bie)白(bai)血(xue)病細(xi)(xi)(xi)(xi)胞(bao)和腺(xian)骨髓轉移的(de)(de)腺(xian)細(xi)(xi)(xi)(xi)胞(bao)，腺(xian)細(xi)(xi)(xi)(xi)胞(bao)呈(cheng)(cheng)陽(yang)(yang)(yang)性(xing)反(fan)(fan)應(ying)(ying)(ying)。口碑好的(de)(de)糖原染色試(shi)劑盒(he)哪家便(bian)宜糖尿病性(xing)腎小球(qiu)硬化癥或(huo)血(xue)管病的(de)(de)診斷(duan)。

糖(tang)(tang)原(yuan)染(ran)色(se)是(shi)病(bing)理學(xue)中(zhong)(zhong)常(chang)規(gui)的(de)染(ran)色(se)方法(fa)之一，McManus在(zai)1946年較(jiao)先使(shi)用(yong)(yong)高(gao)(gao)碘酸-雪夫技術顯示黏(nian)蛋白，該法(fa)常(chang)用(yong)(yong)來顯示糖(tang)(tang)原(yuan)和(he)(he)其他多(duo)糖(tang)(tang)，該染(ran)色(se)試劑(ji)(ji)盒不僅(jin)能(neng)夠顯示糖(tang)(tang)原(yuan)，還(huan)能(neng)顯示中(zhong)(zhong)性(xing)黏(nian)液(ye)性(xing)物(wu)質(zhi)和(he)(he)某些酸性(xing)物(wu)質(zhi)以及(ji)軟骨、垂體、霉菌、色(se)素、淀粉樣物(wu)質(zhi)、基底(di)膜等。過碘酸(又稱高(gao)(gao)碘酸)是(shi)一種(zhong)強氧(yang)(yang)化(hua)(hua)劑(ji)(ji)，它(ta)能(neng)氧(yang)(yang)化(hua)(hua)糖(tang)(tang)類及(ji)有關物(wu)質(zhi)中(zhong)(zhong)的(de)1，2-乙二醇基，使(shi)之變為二醛(quan)，醛(quan)不Schiff試劑(ji)(ji)能(neng)結合成一種(zhong)品(pin)紅化(hua)(hua)合物(wu)，產生紫紅色(se)。由于(yu)高(gao)(gao)碘酸還(huan)可氧(yang)(yang)化(hua)(hua)細胞內其他物(wu)質(zhi)，使(shi)用(yong)(yong)時(shi)應注(zhu)意(yi)選擇(ze)好高(gao)(gao)碘酸濃(nong)度和(he)(he)氧(yang)(yang)化(hua)(hua)時(shi)間，使(shi)氧(yang)(yang)化(hua)(hua)控制(zhi)在(zai)既能(neng)把乙二醇基氧(yang)(yang)化(hua)(hua)成醛(quan)基又不至于(yu)過氧(yang)(yang)化(hua)(hua)，這是(shi)很關鍵的(de)步驟。

粘液(ye)染色法(fa)：粘液(ye)一般有(you)(you)以下幾(ji)點(dian)(dian)特(te)性(xing)(xing)(xing)：（1）對(dui)(dui)鹽基性(xing)(xing)(xing)染料有(you)(you)親合力(li)，因此在HE染色中、切片(pian)上的(de)(de)粘液(ye)呈(cheng)污(wu)穢(hui)藍色。（2）對(dui)(dui)某種(zhong)鹽基性(xing)(xing)(xing)人工合成染料如硫堇或甲苯胺藍有(you)(you)異染性(xing)(xing)(xing)。（3）遇醋(cu)酸發生沉淀，胃粘蛋(dan)白(bai)則除外。（4）溶于弱堿溶液(ye)。常用的(de)(de)粘液(ye)染色有(you)(you)以下幾(ji)種(zhong)：1、P．A．S染色法(fa)：操(cao)作方法(fa)同前(qian)，結(jie)果(guo)；中性(xing)(xing)(xing)粘液(ye)性(xing)(xing)(xing)物質，某些酸性(xing)(xing)(xing)粘液(ye)物質呈(cheng)紅色，核呈(cheng)藍色。2、AB/P.A.S染色法(fa)：該種(zhong)方法(fa)的(de)(de)特(te)點(dian)(dian)是(shi)能同時顯示(shi)出酸性(xing)(xing)(xing)和中性(xing)(xing)(xing)粘液(ye)物質。操(cao)作方法(fa)：（1）切片(pian)常規(gui)脫(tuo)蠟(la)入(ru)水(shui)(shui)。（2）3%醋(cu)酸液(ye)洗(xi)2分鐘(zhong)，入(ru)1%阿(a)利新蘭醋(cu)酸液(ye)（PH2.5）10-20分鐘(zhong)。（3）蒸餾水(shui)(shui)洗(xi)。（4）按P.A.S染色程(cheng)序。（5）蘇(su)木素淡染2-3分鐘(zhong)，水(shui)(shui)洗(xi)。（6）常規(gui)脫(tuo)水(shui)(shui)、封片(pian)。巨幼紅細胞(bao)貧(pin)血(xue)、溶血(xue)性(xing)(xing)(xing)貧(pin)血(xue)、再障的(de)(de)幼紅細胞(bao)PAS染色為陰性(xing)(xing)(xing)。

染色試劑盒(he)：GUS基(ji)(ji)因(yin)(yin)(yin)就是(shi)β-葡(pu)(pu)萄(tao)糖(tang)苷酸(suan)(suan)(suan)(suan)酶(mei)基(ji)(ji)因(yin)(yin)(yin)(β-glucuronidase)，是(shi)從(cong)大(da)腸桿菌K-12菌株分(fen)離(li)出(chu)的(de)(de)(de)一(yi)種酸(suan)(suan)(suan)(suan)性(xing)(xing)水解(jie)(jie)酶(mei)。該基(ji)(ji)因(yin)(yin)(yin)產物(wu)為(wei)β-葡(pu)(pu)萄(tao)糖(tang)苷酸(suan)(suan)(suan)(suan)酶(mei)，屬水解(jie)(jie)酶(mei)類，相(xiang)當穩定(ding)而不(bu)易降解(jie)(jie)，以β-葡(pu)(pu)萄(tao)糖(tang)苷酸(suan)(suan)(suan)(suan)酯類物(wu)質為(wei)底物(wu)，其反應(ying)產物(wu)可(ke)用多(duo)種方法(fa)(fa)檢(jian)測(ce)(ce)出(chu)來。由于(yu)絕大(da)多(duo)數植(zhi)物(wu)沒有(you)檢(jian)測(ce)(ce)到葡(pu)(pu)萄(tao)糖(tang)苷酸(suan)(suan)(suan)(suan)酶(mei)的(de)(de)(de)背景活性(xing)(xing)，因(yin)(yin)(yin)此(ci)這個基(ji)(ji)因(yin)(yin)(yin)被(bei)普遍應(ying)用于(yu)基(ji)(ji)因(yin)(yin)(yin)調控的(de)(de)(de)研究中(zhong)。根(gen)據(ju)GUS基(ji)(ji)因(yin)(yin)(yin)檢(jian)測(ce)(ce)所用的(de)(de)(de)底物(wu)不(bu)同(tong)，可(ke)以選擇三種檢(jian)測(ce)(ce)方法(fa)(fa)：組織化學法(fa)(fa)、分(fen)光(guang)光(guang)度(du)(du)法(fa)(fa)和熒光(guang)法(fa)(fa)（靈感度(du)(du)為(wei)分(fen)光(guang)光(guang)度(du)(du)檢(jian)測(ce)(ce)法(fa)(fa)較高）。其中(zhong)較為(wei)常用的(de)(de)(de)是(shi)組織化學法(fa)(fa)。如(ru)用無水酒精作糖(tang)原的(de)(de)(de)固定(ding)劑，有(you)它的(de)(de)(de)弊病(bing)。福建糖(tang)原染色試劑盒(he)哪(na)家便宜(yi)

切片脫蠟應盡(jin)量干凈，否則影響染色效果。天津如何使用糖原染色試(shi)劑盒量大從(cong)優

糖(tang)(tang)(tang)原染(ran)色(過碘酸-雪夫(fu)反(fan)應(ying))原理多糖(tang)(tang)(tang)中乙二醇基(ji)經過碘酸氧化(hua)成(cheng)(cheng)二醛基(ji)與(yu)無色品紅(hong)(hong)結合，形成(cheng)(cheng)紫紅(hong)(hong)色化(hua)合物(wu)。沉積于含(han)(han)糖(tang)(tang)(tang)原的(de)(de)胞(bao)(bao)漿中。結果：胞(bao)(bao)漿中出(chu)現彌(mi)散(san)狀(zhuang)(zhuang)，顆粒狀(zhuang)(zhuang)，塊狀(zhuang)(zhuang)紅(hong)(hong)色為陽(yang)(yang)性(xing)。無紅(hong)(hong)色顆粒為陰性(xing)_x005f_x000c_臨床意義1、正(zheng)常血(xue)細(xi)(xi)胞(bao)(bao)的(de)(de)染(ran)色反(fan)應(ying)粒系(xi)：原粒細(xi)(xi)胞(bao)(bao)糖(tang)(tang)(tang)原含(han)(han)量很低，隨著細(xi)(xi)胞(bao)(bao)的(de)(de)分化(hua)成(cheng)(cheng)熟，糖(tang)(tang)(tang)原含(han)(han)量逐增加，至成(cheng)(cheng)熟階段糖(tang)(tang)(tang)原含(han)(han)量十分豐富。淋(lin)(lin)巴細(xi)(xi)胞(bao)(bao)：糖(tang)(tang)(tang)原常凝(ning)聚成(cheng)(cheng)顆粒或(huo)塊狀(zhuang)(zhuang)。巨(ju)核(he)細(xi)(xi)胞(bao)(bao)－血(xue)小板系(xi)統(tong)：PAS反(fan)應(ying)呈粗(cu)大的(de)(de)紫色顆粒或(huo)團塊。正(zheng)常紅(hong)(hong)系(xi)：陰性(xing)。對疾(ji)病診斷的(de)(de)意義白血(xue)病類型的(de)(de)鑒(jian)別：急性(xing)粒細(xi)(xi)胞(bao)(bao)白血(xue)病：原始(shi)粒細(xi)(xi)胞(bao)(bao)呈陰性(xing)或(huo)弱陽(yang)(yang)性(xing)反(fan)應(ying)，以彌(mi)散(san)性(xing)為主；急性(xing)淋(lin)(lin)巴細(xi)(xi)胞(bao)(bao)白血(xue)病原、幼淋(lin)(lin)巴細(xi)(xi)胞(bao)(bao)多呈粗(cu)顆粒、塊狀(zhuang)(zhuang)陽(yang)(yang)性(xing)反(fan)應(ying)天(tian)津如何使用(yong)糖(tang)(tang)(tang)原染(ran)色試劑(ji)盒量大從優

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