產品(pin)庫(ku)(ku)科(ke)研資訊實驗(yan)(yan)試用(yong)中心技術專題會議商城生(sheng)(sheng)(sheng)意通品(pin)牌求購發布產品(pin)儀(yi)(yi)器(qi)(qi)設(she)(she)(she)備(bei)庫(ku)(ku)細(xi)胞(bao)分(fen)(fen)(fen)析儀(yi)(yi)器(qi)(qi)儲存(cun)保存(cun)設(she)(she)(she)備(bei)實驗(yan)(yan)室(shi)箱體/搖床(chuang)實驗(yan)(yan)室(shi)安全設(she)(she)(she)備(bei)生(sheng)(sheng)(sheng)物(wu)芯(xin)片(pian)3D打印儀(yi)(yi)器(qi)(qi)設(she)(she)(she)備(bei)庫(ku)(ku)生(sheng)(sheng)(sheng)物(wu)芯(xin)片(pian)影像系(xi)統(tong)測(ce)讀系(xi)統(tong)光(guang)譜(pu)系(xi)統(tong)分(fen)(fen)(fen)子生(sheng)(sheng)(sheng)物(wu)實驗(yan)(yan)儀(yi)(yi)器(qi)(qi)顯微(wei)(wei)系(xi)統(tong)色(se)譜(pu)系(xi)統(tong)質譜(pu)系(xi)統(tong)實驗(yan)(yan)室(shi)自動(dong)化基因組(zu)/蛋白(bai)組(zu)設(she)(she)(she)備(bei)植物(wu)生(sheng)(sheng)(sheng)理(li)/動(dong)物(wu)生(sheng)(sheng)(sheng)理(li)毒理(li)/實驗(yan)(yan)動(dong)物(wu)設(she)(she)(she)備(bei)神經生(sheng)(sheng)(sheng)物(wu)學(xue)儀(yi)(yi)器(qi)(qi)組(zu)織(zhi)學(xue)設(she)(she)(she)備(bei)過(guo)濾裝置電化學(xue)儀(yi)(yi)器(qi)(qi)細(xi)胞(bao)培(pei)養儀(yi)(yi)器(qi)(qi)耗(hao)(hao)材(cai)(cai)(cai)庫(ku)(ku)常(chang)用(yong)耗(hao)(hao)材(cai)(cai)(cai)移液器(qi)(qi)(Pipette)PCR耗(hao)(hao)材(cai)(cai)(cai)儀(yi)(yi)器(qi)(qi)配(pei)套耗(hao)(hao)材(cai)(cai)(cai)細(xi)胞(bao)/組(zu)織(zhi)培(pei)養耗(hao)(hao)材(cai)(cai)(cai)分(fen)(fen)(fen)子生(sheng)(sheng)(sheng)物(wu)學(xue)耗(hao)(hao)材(cai)(cai)(cai)耗(hao)(hao)材(cai)(cai)(cai)庫(ku)(ku)常(chang)用(yong)耗(hao)(hao)材(cai)(cai)(cai)移液器(qi)(qi)(Pipette)吸頭(Tips)瓶(ping)(Bottle)瓶(ping)(Flask)管(Tube&Vial)PCR耗(hao)(hao)材(cai)(cai)(cai)微(wei)(wei)孔板色(se)譜(pu)柱色(se)譜(pu)介質細(xi)胞(bao)/組(zu)織(zhi)培(pei)養耗(hao)(hao)材(cai)(cai)(cai)分(fen)(fen)(fen)子生(sheng)(sheng)(sheng)物(wu)學(xue)耗(hao)(hao)材(cai)(cai)(cai)過(guo)濾耗(hao)(hao)材(cai)(cai)(cai)儀(yi)(yi)器(qi)(qi)配(pei)套耗(hao)(hao)材(cai)(cai)(cai)試劑庫(ku)(ku)細(xi)胞(bao)培(pei)養細(xi)胞(bao)干細(xi)胞(bao)免疫檢(jian)測(ce)/ELISA常(chang)用(yong)生(sheng)(sheng)(sheng)化試劑酶試劑庫(ku)(ku)生(sheng)(sheng)(sheng)物(wu)分(fen)(fen)(fen)子常(chang)用(yong)生(sheng)(sheng)(sheng)化試劑酶蛋白(bai)質/抗原/多肽cDNA及(ji)合成(cheng)純化PCR/RT-PCR/qPCR載體及(ji)構建文庫(ku)(ku)及(ji)構建克隆(long)與(yu)表達表達分(fen)(fen)(fen)析核(he)(he)酸(suan)/蛋白(bai)合成(cheng)核(he)(he)酸(suan)分(fen)(fen)(fen)析核(he)(he)酸(suan)檢(jian)測(ce)核(he)(he)酸(suan)純化RNAi技術(siRNA,miRNA。動(dong)物(wu)模型的表現與(yu)人類(lei)疾病(bing)可能存(cun)在差異，因此(ci)需要謹慎使用(yong)。湖南疾病(bing)科(ke)研技術服務分(fen)(fen)(fen)離

應根據(ju)所檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)指標(biao)的(de)(de)(de)(de)要求(qiu)，需采取(qu)不同(tong)策略處理。在如今分(fen)子(zi)(zi)學(xue)當(dang)道(dao)的(de)(de)(de)(de)現實(shi)(shi)(shi)背(bei)景(jing)下(xia)，動物(wu)(wu)實(shi)(shi)(shi)驗除(chu)了對(dui)(dui)實(shi)(shi)(shi)驗動物(wu)(wu)的(de)(de)(de)(de)體征及(ji)(ji)生(sheng)理指標(biao)進(jin)行(xing)全的(de)(de)(de)(de)監控外(wai)(wai)，各(ge)種分(fen)子(zi)(zi)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)甚至(zhi)是組學(xue)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)也開始大行(xing)其道(dao)，動物(wu)(wu)實(shi)(shi)(shi)驗結束之(zhi)后的(de)(de)(de)(de)機制(zhi)研(yan)究(jiu)成為了實(shi)(shi)(shi)驗的(de)(de)(de)(de)重(zhong)頭戲。一般(ban)來說(shuo)，動物(wu)(wu)實(shi)(shi)(shi)驗檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)對(dui)(dui)象主要包(bao)括(kuo)：（1）體液中(zhong)(zhong)(zhong)各(ge)類(lei)(lei)因子(zi)(zi)定(ding)(ding)性(xing)(xing)及(ji)(ji)定(ding)(ding)量檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)由于(yu)此(ci)類(lei)(lei)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)對(dui)(dui)象多為蛋(dan)白及(ji)(ji)各(ge)類(lei)(lei)小分(fen)子(zi)(zi)物(wu)(wu)質(zhi)，因此(ci)在取(qu)樣過程中(zhong)(zhong)(zhong)應注意防(fang)止此(ci)類(lei)(lei)物(wu)(wu)質(zhi)降解(jie)，在獲(huo)取(qu)后，應及(ji)(ji)時置于(yu)溫(wen)（≤-80℃）進(jin)行(xing)保存，在后續實(shi)(shi)(shi)驗過程中(zhong)(zhong)(zhong)，也應當(dang)注意保存條件的(de)(de)(de)(de)穩定(ding)(ding)性(xing)(xing)，避免反復(fu)凍融。常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)方(fang)法(fa)便是酶(mei)聯(lian)免吸附(fu)測(ce)(ce)(ce)(ce)定(ding)(ding)（ELISA），除(chu)此(ci)之(zhi)外(wai)(wai)，可利(li)用(yong)(yong)(yong)(yong)生(sheng)化(hua)分(fen)析儀及(ji)(ji)不同(tong)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)方(fang)法(fa)的(de)(de)(de)(de)（比(bi)色法(fa)、比(bi)濁法(fa)等）方(fang)法(fa)對(dui)(dui)各(ge)類(lei)(lei)生(sheng)化(hua)指標(biao)及(ji)(ji)因子(zi)(zi)進(jin)行(xing)定(ding)(ding)性(xing)(xing)及(ji)(ji)定(ding)(ding)量檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)。（2）生(sheng)理變化(hua)及(ji)(ji)免組化(hua)檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)的(de)(de)(de)(de)理檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)多使用(yong)(yong)(yong)(yong)H&E染(ran)(ran)色對(dui)(dui)細(xi)(xi)胞(bao)(bao)質(zhi)及(ji)(ji)細(xi)(xi)胞(bao)(bao)核進(jin)行(xing)染(ran)(ran)色標(biao)記(ji)，以觀察細(xi)(xi)胞(bao)(bao)變化(hua)。除(chu)此(ci)之(zhi)外(wai)(wai)，許多特殊染(ran)(ran)色也在理生(sheng)理變化(hua)中(zhong)(zhong)(zhong)得到(dao)了應用(yong)(yong)(yong)(yong)，如利(li)用(yong)(yong)(yong)(yong)Masson染(ran)(ran)色檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)纖維化(hua)變，Nissl染(ran)(ran)色檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)神經(jing)元(yuan)損(sun)傷，β-半乳(ru)糖甘酶(mei)染(ran)(ran)色檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)細(xi)(xi)胞(bao)(bao)衰老等。此(ci)外(wai)(wai)，還可以通過免組化(hua)技(ji)術對(dui)(dui)某些特異性(xing)(xing)標(biao)記(ji)物(wu)(wu)進(jin)行(xing)免顯(xian)色檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)，達到(dao)原位定(ding)(ding)性(xing)(xing)或定(ding)(ding)量檢(jian)(jian)(jian)測(ce)(ce)(ce)(ce)的(de)(de)(de)(de)目的(de)(de)(de)(de)。。黑龍江(jiang)外(wai)(wai)包(bao)科研(yan)技(ji)術服務實(shi)(shi)(shi)驗常(chang)(chang)(chang)用(yong)(yong)(yong)(yong)于(yu)研(yan)究(jiu)細(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)成瘤能力、細(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)轉移、細(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)耐藥(yao)和靶分(fen)子(zi)(zi)對(dui)(dui)腫瘤細(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)發(fa)展的(de)(de)(de)(de)影(ying)響等等。

METTL3能(neng)夠促(cu)(cu)進(jin)肺腺細(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)生(sheng)長、生(sheng)存(cun)(cun)和(he)侵(qin)襲，但還(huan)不(bu)清楚它(ta)是否作(zuo)為m6A調(diao)節(jie)器或(huo)效應器發揮(hui)作(zuo)用[25]。在急性髓細(xi)(xi)胞(bao)(bao)白血病(bing)（AML）患者中(zhong)，m(6)A調(diao)控(kong)基(ji)(ji)因(yin)(yin)的(de)(de)(de)(de)突變或(huo)拷貝(bei)數變化(hua)與TP53突變存(cun)(cun)在密切聯系，且(qie)m(6)A調(diao)控(kong)基(ji)(ji)因(yin)(yin)的(de)(de)(de)(de)改變與AML不(bu)良(liang)預相關(guan)(guan)[26]。此外(wai)，FTO在AML中(zhong)高(gao)表達(da)(da)(da)，它(ta)通過(guo)(guo)降低mRNA轉錄本中(zhong)的(de)(de)(de)(de)m(6)水平(ping)，調(diao)節(jie)ASB2和(he)RARA等靶點的(de)(de)(de)(de)表達(da)(da)(da)，增強了白血病(bing)基(ji)(ji)因(yin)(yin)介(jie)導(dao)(dao)的(de)(de)(de)(de)細(xi)(xi)胞(bao)(bao)轉化(hua)和(he)白血病(bing)形(xing)(xing)(xing)成(cheng)，并抑(yi)制全(quan)反式維甲(jia)酸(ATRA)誘(you)導(dao)(dao)的(de)(de)(de)(de)AML細(xi)(xi)胞(bao)(bao)分(fen)化(hua)[27]。在脂肪形(xing)(xing)(xing)成(cheng)過(guo)(guo)程(cheng)中(zhong)，FTO表達(da)(da)(da)與m6A水平(ping)成(cheng)負相關(guan)(guan)，促(cu)(cu)進(jin)脂肪形(xing)(xing)(xing)成(cheng)[3]。在膠(jiao)質(zhi)(zhi)細(xi)(xi)胞(bao)(bao)瘤(liu)樣細(xi)(xi)胞(bao)(bao)中(zhong)，ALKBH5通過(guo)(guo)lncRNAFOXM1介(jie)導(dao)(dao)FOXM1基(ji)(ji)因(yin)(yin)pre-mRNA上的(de)(de)(de)(de)m6A修(xiu)(xiu)(xiu)飾維持膠(jiao)質(zhi)(zhi)瘤(liu)細(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)成(cheng)瘤(liu)性[28]。此外(wai)，甲(jia)基(ji)(ji)轉移酶(mei)METTL3或(huo)METTL14的(de)(de)(de)(de)敲除(chu)，能(neng)夠改變m6A的(de)(de)(de)(de)富(fu)集(ji)和(he)ADAM19的(de)(de)(de)(de)表達(da)(da)(da)，極大地(di)促(cu)(cu)進(jin)了膠(jiao)質(zhi)(zhi)瘤(liu)細(xi)(xi)胞(bao)(bao)的(de)(de)(de)(de)生(sheng)長、自我更新(xin)和(he)形(xing)(xing)(xing)成(cheng)[29]。圖2m6ARNA修(xiu)(xiu)(xiu)飾和(he)介(jie)導(dao)(dao)的(de)(de)(de)(de)功(gong)(gong)能(neng)[30]m6A的(de)(de)(de)(de)研(yan)究(jiu)方向主要是通過(guo)(guo)研(yan)究(jiu)m6A修(xiu)(xiu)(xiu)飾相關(guan)(guan)的(de)(de)(de)(de)甲(jia)基(ji)(ji)化(hua)、去甲(jia)基(ji)(ji)化(hua)酶(mei)和(he)識別蛋白的(de)(de)(de)(de)功(gong)(gong)能(neng)，進(jin)而研(yan)究(jiu)m6A修(xiu)(xiu)(xiu)飾的(de)(de)(de)(de)生(sheng)物學(xue)功(gong)(gong)能(neng)和(he)作(zuo)用機制：一(yi)般通過(guo)(guo)敲除(chu)m6A酶(mei)分(fen)子，研(yan)究(jiu)下游(you)功(gong)(gong)能(neng)基(ji)(ji)因(yin)(yin)分(fen)子的(de)(de)(de)(de)表達(da)(da)(da)和(he)m6A甲(jia)基(ji)(ji)化(hua)情(qing)況，通過(guo)(guo)介(jie)導(dao)(dao)相關(guan)(guan)基(ji)(ji)因(yin)(yin)異常（可(ke)變剪切、穩定性、翻譯、miRNA調(diao)控(kong)）影響細(xi)(xi)胞(bao)(bao)表型和(he)功(gong)(gong)能(neng)特(te)征。

|基(ji)(ji)(ji)因(yin)組DNA擴(kuo)增(zeng)(zeng)|RNA擴(kuo)增(zeng)(zeng)|克(ke)隆(long)(long)與(yu)表(biao)達克(ke)隆(long)(long)基(ji)(ji)(ji)因(yin)|克(ke)隆(long)(long)試(shi)(shi)(shi)劑盒(he)(he)|克(ke)隆(long)(long)篩選|感受態細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)|突變轉(zhuan)染|轉(zhuan)化(hua)|體(ti)(ti)外(wai)轉(zhuan)錄/翻(fan)譯|其它(ta)(ta)表(biao)達分(fen)(fen)析(xi)Northern印跡分(fen)(fen)析(xi)|分(fen)(fen)支DNA和mRNA定(ding)量(liang)|差別基(ji)(ji)(ji)因(yin)表(biao)達|反義寡(gua)核苷(gan)酸|RACE試(shi)(shi)(shi)劑盒(he)(he)|SAGE試(shi)(shi)(shi)劑盒(he)(he)|其它(ta)(ta)抗(kang)(kang)體(ti)(ti)蛋(dan)白(bai)(bai)(bai)A,GPLUS|抑(yi)(yi)制/凋亡抗(kang)(kang)體(ti)(ti)|信(xin)號分(fen)(fen)子(zi)抗(kang)(kang)體(ti)(ti)結構蛋(dan)白(bai)(bai)(bai)抗(kang)(kang)體(ti)(ti)|磷酸化(hua)特(te)異抗(kang)(kang)體(ti)(ti)|融合(he)蛋(dan)白(bai)(bai)(bai)tag抗(kang)(kang)體(ti)(ti)非哺乳(ru)動物(wu)(wu)蛋(dan)白(bai)(bai)(bai)抗(kang)(kang)體(ti)(ti)|細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)周期蛋(dan)白(bai)(bai)(bai)抗(kang)(kang)體(ti)(ti)|轉(zhuan)錄調節蛋(dan)白(bai)(bai)(bai)抗(kang)(kang)體(ti)(ti)|抗(kang)(kang)體(ti)(ti)制備佐劑|抗(kang)(kang)體(ti)(ti)片段|抗(kang)(kang)體(ti)(ti)同種型|抗(kang)(kang)體(ti)(ti)純化(hua)|抗(kang)(kang)體(ti)(ti)制備試(shi)(shi)(shi)劑盒(he)(he)|其它(ta)(ta)第二(er)抗(kang)(kang)體(ti)(ti)westernblot二(er)抗(kang)(kang)|免(mian)疫組化(hua)二(er)抗(kang)(kang)|其它(ta)(ta)試(shi)(shi)(shi)劑盒(he)(he)ELISA試(shi)(shi)(shi)劑盒(he)(he)|免(mian)疫組化(hua)試(shi)(shi)(shi)劑盒(he)(he)|放射免(mian)疫試(shi)(shi)(shi)劑盒(he)(he)|westernblot試(shi)(shi)(shi)劑盒(he)(he)|流(liu)式細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)試(shi)(shi)(shi)劑盒(he)(he)|其它(ta)(ta)抗(kang)(kang)體(ti)(ti)相關(guan)siRNA|重組蛋(dan)白(bai)(bai)(bai)|白(bai)(bai)(bai)蛋(dan)白(bai)(bai)(bai)|試(shi)(shi)(shi)劑|其它(ta)(ta)分(fen)(fen)子(zi)生(sheng)物(wu)(wu)學(xue)(xue)服(fu)(fu)務(wu)DNA提取/純化(hua)|DNA測(ce)序|第二(er)代高通(tong)量(liang)測(ce)序|DNA保存和活化(hua)|基(ji)(ji)(ji)因(yin)合(he)成(cheng)|基(ji)(ji)(ji)因(yin)步行|基(ji)(ji)(ji)因(yin)組分(fen)(fen)析(xi)生(sheng)物(wu)(wu)信(xin)息學(xue)(xue)|SNP檢(jian)(jian)測(ce)|實(shi)(shi)時定(ding)量(liang)PCR服(fu)(fu)務(wu)|文庫/載體(ti)(ti)構建|寡(gua)核苷(gan)酸合(he)成(cheng)Oligo合(he)成(cheng)|實(shi)(shi)時PCROligo合(he)成(cheng)|其它(ta)(ta)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)生(sheng)物(wu)(wu)學(xue)(xue)服(fu)(fu)務(wu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)培養(yang)|流(liu)式細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)檢(jian)(jian)測(ce)|細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)毒(du)性測(ce)試(shi)(shi)(shi)|細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)因(yin)子(zi)生(sheng)物(wu)(wu)檢(jian)(jian)測(ce)|影像服(fu)(fu)務(wu)|篩選/發育服(fu)(fu)務(wu)|其它(ta)(ta)干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)技(ji)術服(fu)(fu)務(wu)干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)提取|干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)鑒定(ding)|干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)誘導分(fen)(fen)化(hua)|干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)常規檢(jian)(jian)測(ce)|干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)擴(kuo)增(zeng)(zeng)|干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)轉(zhuan)基(ji)(ji)(ji)因(yin)|干(gan)(gan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)其他相關(guan)實(shi)(shi)驗|微生(sheng)物(wu)(wu)學(xue)(xue)服(fu)(fu)務(wu)微生(sheng)物(wu)(wu)鑒定(ding)|抑(yi)(yi)菌作用(yong)測(ce)試(shi)(shi)(shi)|內(nei)測(ce)試(shi)(shi)(shi)|內(nei)。隨著跨學(xue)(xue)科研(yan)究(jiu)的深入開展，動物(wu)(wu)疾病模型將在(zai)未來發揮更為普(pu)遍的作用(yong)，成(cheng)為生(sheng)命科學(xue)(xue)、醫學(xue)(xue)等領域研(yan)究(jiu)工具。

小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)尾(wei)尖(jian)用(yong)酒精擦拭尾(wei)巴，引(yin)起輕(qing)(qing)(qing)(qing)微(wei)(wei)的(de)(de)(de)血(xue)(xue)(xue)管(guan)擴(kuo)張；用(yong)無菌手(shou)(shou)術刀(dao)、刀(dao)片(pian)或(huo)鋒(feng)利(li)的(de)(de)(de)剪刀(dao)，快速截斷(duan)小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)尾(wei)尖(jian)1-2毫米。如果需(xu)要多次(ci)，之(zhi)后(hou)每次(ci)需(xu)截除2-3毫米；從(cong)尾(wei)部像(xiang)尾(wei)尖(jian)方(fang)向按(an)摩，增加血(xue)(xue)(xue)流；用(yong)采集血(xue)(xue)(xue)液(ye)；結束后(hou)，按(an)壓傷口或(huo)使(shi)用(yong)止血(xue)(xue)(xue)劑來止血(xue)(xue)(xue)；每次(ci)量大(da)約(yue)可達(da)。小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)眼(yan)(yan)(yan)球(qiu)取(qu)(qu)血(xue)(xue)(xue)單(dan)手(shou)(shou)保定(ding)(ding)(ding)好小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)；必(bi)要時(shi)剪掉小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)胡須，防止污染(ran)血(xue)(xue)(xue)液(ye)；輕(qing)(qing)(qing)(qing)壓取(qu)(qu)血(xue)(xue)(xue)側(ce)(ce)眼(yan)(yan)(yan)部皮膚，使(shi)眼(yan)(yan)(yan)球(qiu)充血(xue)(xue)(xue)突出；用(yong)彎頭鑷夾取(qu)(qu)眼(yan)(yan)(yan)球(qiu)并快速在(zai)摘(zhai)取(qu)(qu)；同時(shi)用(yong)左手(shou)(shou)中(zhong)(zhong)指(zhi)輕(qing)(qing)(qing)(qing)按(an)小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)心臟部位，以加快心臟泵(beng)血(xue)(xue)(xue)速度(du)；當(dang)血(xue)(xue)(xue)液(ye)流盡時(shi)，用(yong)脫臼法處死小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)；大(da)鼠(shu)(shu)(shu)(shu)眼(yan)(yan)(yan)眶(kuang)取(qu)(qu)血(xue)(xue)(xue)先將(jiang)小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)進(jin)行麻(ma)醉，大(da)鼠(shu)(shu)(shu)(shu)翻(fan)正反射(she)消失(shi)時(shi)不在(zai)繼續麻(ma)醉；抗凝處理過的(de)(de)(de)玻璃毛細(xi)(xi)采樣(yang)管(guan)，掰成2-3厘米長度(du)；右手(shou)(shou)食指(zhi)和拇指(zhi)輕(qing)(qing)(qing)(qing)按(an)眼(yan)(yan)(yan)眶(kuang)兩側(ce)(ce)皮膚，使(shi)眼(yan)(yan)(yan)球(qiu)突出，毛細(xi)(xi)管(guan)從(cong)內側(ce)(ce)的(de)(de)(de)眼(yan)(yan)(yan)球(qiu)與眼(yan)(yan)(yan)瞼的(de)(de)(de)縫隙處進(jin)入，輕(qing)(qing)(qing)(qing)輕(qing)(qing)(qing)(qing)旋轉毛細(xi)(xi)管(guan)，稍微(wei)(wei)深入眼(yan)(yan)(yan)球(qiu)后(hou)上(shang)方(fang)，血(xue)(xue)(xue)液(ye)流速快的(de)(de)(de)時(shi)候4-5滴即可，溫柔的(de)(de)(de)將(jiang)毛細(xi)(xi)管(guan)取(qu)(qu)出；用(yong)棉簽按(an)壓大(da)鼠(shu)(shu)(shu)(shu)眼(yan)(yan)(yan)眶(kuang)止血(xue)(xue)(xue)，每次(ci)可取(qu)(qu)血(xue)(xue)(xue)50-100微(wei)(wei)升(sheng)，將(jiang)血(xue)(xue)(xue)液(ye)放(fang)入冰盒中(zhong)(zhong)保存(cun)。小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)心臟取(qu)(qu)血(xue)(xue)(xue)先將(jiang)小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)麻(ma)醉，稍靠右側(ce)(ce)平躺在(zai)手(shou)(shou)術板上(shang)固定(ding)(ding)(ding)；左側(ce)(ce)第(di)三(san)四根肋(lei)骨間觸摸心臟，跳(tiao)動明顯(xian)，慢(man)慢(man)進(jin)針(zhen)；針(zhen)有(you)回血(xue)(xue)(xue)停止進(jin)針(zhen)，開始(shi)取(qu)(qu)血(xue)(xue)(xue)；一次(ci)可以300到(dao)500微(wei)(wei)升(sheng)，小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)存(cun)活，放(fang)到(dao)加熱墊(dian)上(shang)待小(xiao)(xiao)(xiao)(xiao)(xiao)鼠(shu)(shu)(shu)(shu)蘇醒后(hou)放(fang)入籠中(zhong)(zhong)。細(xi)(xi)胞劃痕(wound healing）法是(shi)簡(jian)捷測定(ding)(ding)(ding)細(xi)(xi)胞遷(qian)移運(yun)動和修復能力的(de)(de)(de)方(fang)法。云南疾(ji)病科(ke)研(yan)技術服(fu)務(wu)培(pei)養

希望(wang)能給大家提供到幫助，了解(jie)更(geng)多關于(yu)ELISA試劑盒的問題歡迎(ying)來(lai)電咨詢。湖南疾(ji)病科研技術(shu)服務分(fen)離

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